Part:BBa_K2165003
Violacein D gene with Gal1 promoter + ADH1 terminator
The promoter for the biobrick uses the Gal1 promoter sequence Part:BBa_J63006, which is activated in the presence of galactose but repressed in the presence of glucose. VioD BBa_K2165001 was used as the CDS. The biobrick ends with the ADH1 terminator BBa_K801012 for yeast that was submitted by the TU Munich team in 2012.
This biobrick was designed to characterize the codon optimized version of VioD. The codon optimized VioD should have higher translation rates than the previously made VioD, and this plasmid construct will be used to determine the change in the expression levels for the pathway. This composite part was also designed for use in the University of Washington 2016 iGEM team's chemostat-autoinjector proof of concept. For more information on the role of VioD in the violacein pathway see part BBa_K2165001.
Usage and Biology
It should be stressed that without the other main components of the Violacein pathway (VioA, VioB, and VioE, see part BBa_K726015 as an example), the substrate for VioC and VioD will not be produced and no pigment will be visible.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 150
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1177
Illegal SapI.rc site found at 1558
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